Composite

Part:BBa_M1193:Design

Designed by: S. Gabrielle Gladstone   Group: UtahState_BE5930_-_S11   (2013-03-25)


Gabi's part in GFP assay


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2158
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 625
    Illegal BglII site found at 640
    Illegal BglII site found at 1044
    Illegal XhoI site found at 3634
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1730
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3193
    Illegal SapI.rc site found at 266


Design Notes

I overwrote this dummy part to save part numbers for others in our group, M163(X).

This is my fusion protein with Chauncey's bioluminescence part, M1635, along with the expression machinery and LacO.

Source

J23119 (promoter) B0034 (RBS) M1632 (Gabi's fusion protein) K23304 (LacO so that the fusion protein can bind) M1635 (Chauncey's bioluminescence) B0010 (stop)

References